Department of Molecular Biology & Biochemistry spacer Department of Genetics

Introduction to Molecular Biology & Biochemistry Research 694:315 &
Introduction to Research in Genetics 447:315

Lecture: T3
Tues 12:00-1:20
Waksman Auditorium
(directions)



Labs:
02: Tues 1:40-6:20
03: Wed 1:40-6:20
Waksman, Room 19

Spring 2010

IntroductionIndependent Undergraduate ResearchSummer Fellowships
DNA Sequence Analysis Program Published Sequences

Course Instructors:
Dr. Monica Driscoll  
  Richard Padgett  
Bryce Nickels  
Lab Coordinator:
Janet Mead  
Teaching Assistants:
   
   

Summary: This course is designed to give students the theoretical background and practical experience with many of the techniques that will likely be used during an undergraduate research project.  We will also provide assistance to help students identify research labs that they may be interested in. After some initial training in several techniques used in molecular biology, students will start to work on their main research project.  The project involves sequencing random clones from a cDNA library.  The sequences derived from these experiments will be used to query a database for sequence similarity using the appropriate computer software.  When matches are found, students will then search the scientific literature for information on the genes that they have identified.   Students in the course will identify genes that have never been sequenced before and students will have the opportunity to publish their sequence on GenBank the international sequence database of the National Center of Biotechnology Information (NCBI). For more information on the course click here

Lecture & Lab Schedule
Week
Date
Topic
1
1/19
Lecture: Introduction, The Basics | Translation movie
 
1/19-1/20
Lab: Introduction and Rules, pipeting and plating
2
1/26
Lecture: Vectors, Libraries, Plasmid Minipreps
 
1/26-1/27
Lab: Basic Techniques
3
2/02
Lecture: Analyzing DNA - Restriction enzymes & PCR, Mapping
Animations: Restriction Digests, PCR, ELectrophoresis
 
2/2-2/3
Lab: Plasmid DNA Minipreps
4
2/9
Lecture: Sequencing DNA (BN) DNA Sequencing: Bench to bedside and beyond
 
2/9/-2/10
Lab: Restriction Digests & PCR
5
2/16
Lecture: Bioinformatics Analysis Part I: Crop and edit DNA sequences
 
2/16-2/17
Lab: Gel Electrophoresis (Tutorial.pdf)
6
2/23
Lecture: Bioinformatics Analysis Part II: BLASTN, BLASTX,
 
2/23-2/24
Lab: DNA Sequencing, Minipreps
7
3/02
Lecture: Bioinformatics Analysis Part III: ORF, BLASTP
 
3/2-3/3
Lab: Restriction Digests & Gel Electrophoresis
8
3/9
Lecture: In Class Exam (12:00 - 1:20 PM)
 
3/9-3/10
Lab: DNA Sequencing, Analysis of PC clones
9
3/16
Spring Break - No Class
10
3/23
Lecture: Bioinformatics Analysis Part IV: , Lit search, Clustal and Structure Cn3D
 
3/23-3/24
Lab: Sequence Analysis DUE: Notebook & Clone Management Sheet
11
3/30
Lecture: RNAi Fire 1998 RNAi movie Grobhans 2008
 
3/30-3/31
Lab: Sequence Analysis: Analyze all clones to identify a gene of interest for the presentation
12
4/06
Lecture: Tools of Molecular Biology
 
4/6-4/7
Lab: Sequence Analysis, DUE: Gene of Interest Summary
13
4/13
Lecture: What to do next with C. elegans Salvation of Doug; Demise of Bill; Checks & Balances;
 
4/13-14/14
Lab: Sequence Analysis, DUE: Outline of Presentation and Abstract of a Primary Article
14
4/20
Lecture: Approaches in Higher Eukaryotes; Giving Presentations
 
4/20-4/21
Lab: DUE: Completion of the analysis of all the unknown clones
15
4/27
Lecture: Careers in Biomedical Sciences,
 
4/27-4/28
Lab: Presentations, DUE: Abstract of the Presentation
DUE: All Powerpoint files are due in the Sakai Dropbox by 9:00 AM on the day of your lab
16
??
Final Exam

last updated 8/24/2009