Week |
Date |
Topic |
|
9/1 |
No Class! |
|
9/8 |
No Class! This day is scheduled as a Monday |
1 |
9/15 |
Lecture: Introduction; Project; Why is transcription important for biology and human diseases?
Review of of the general process of transcription and reporter genes |
| |
|
Lab 0: Lab Introduction and Rules, |
| |
|
Lab 1A: Pipeting exercises |
| |
|
Lab 1B: Plating the mutant plasmid library transformed into yeast |
2 |
9/22 |
Lecture: Why do we work in yeast? Review of yeast biology and genetics |
| |
|
Lab 2A: Pipeting Dilutions, DNA concentration |
| |
|
Lab 2B: Yeast Streakouts |
3 |
9/29 |
Lecture: Eukaryotic chromatin structure and the role of Sir2 in transcriptional silencing |
| |
|
Lab 3: Setting up yeast ON cultures for silencing spotting assay |
4 |
10/6 |
Lecture 4: Vectors and DNA minpreps |
| |
|
Lab 4A: Set up transcriptional silencing spot assay |
| |
10/9 |
Lab 4B: Setting up yeast ON cultures for plasmid rescue experiments (Note this is on a Friday) |
5 |
10/13 |
Lecture 5: Analyzing DNA - Restriction enzymes & PCR, and gel electrophoresis
Animations: Restriction Digests, PCR, Electrophoresis |
| |
|
Lab 5A: Plasmid DNA purification from yeast |
6 |
10/20 |
Lecture 6: DNA sequencing and databases, 4-Peaks, FinchTV |
| |
|
Lab 6A: Transform bacteria with plasmid DNA |
| |
10/26 |
Lab 6B: Set up bacterial ON cultures (Note this is on a Monday) |
7 |
10/27 |
Lecture 7: Genetic analysis of transcriptional regulatory sites; Genetic screens for activator and repressor proteins. |
| |
|
Lab 7A: Plasmid DNA purification from bacteria |
| |
|
Lab 7B: Set up restriction digests & PCR |
8 |
11/3 |
Lecture 8: Silencing vs repression, Sir2 vs Hst1; PCR Mutagenesis and screen of the HST1:SIR2 chimera |
| |
|
Lab 8A: Agarose gel electrophoresis of plasmid restrictiion digests and PCR |
| |
|
Lab 8B: DNA Sequencing |
9 |
11/10 |
Lecture: In Class Exam |
| |
|
Lab 9A: Transformation of a yeast strain with the purified mutant plasmid |
| |
11/13 |
Lab 9B: Streakout of yeast cultures (Note this is on a Friday) |
10 |
11/17 |
Lecture 10: DNA Sequence analysis, Make a contig; Identify the mutants,
|
| |
|
Lab 10: LacZ filter assays for transcriptional repression by the mutants |
11 |
11/24 |
Lecture 11: Principals of protein structure, Review of Sir2 structure; Structure modeling using CN3D or RasMol Results, problems and interpretations
|
| |
|
Lab 11: DNA sequence analysis of the mutant plasmids |
12 |
12/1 |
Lecture 12: Giving Presentations, Finding a Lab, Careers in Biomedical Sciences |
| |
|
Lab 12: Molecular modeling of the mutant proteins |
13 |
12/8 |
Lecture & Lab: Presentations DUE: Powerpoint files are due for all students by 9:00 AM |
|
12/16 |
Exam 12:00 noon - 3:00 PM |
