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Freshman Seminar: Control of Gene Expression 090:101 Section XX

Lecture/Lab: M5
Mon 1:40-3:00 PM
Waksman,
Discussions: Rm 311
Lab: Rm 019
(Directions)

Fall 2008
Course Instructor Dr. Andrew Vershon (732) 445-2905

Announcements:


Lecture Schedule
Week
 Date Discussion Topic/Lab Notes/Labs Figures
1
 9/8

Discussions: Course Introduction
Why is transcription important for biology and human diseases?
Review of of the general process of transcription
Why do we work in yeast? Review of Yeast Biology

Transcription
Editorial

 L1.ppt
2
 9/15

Discussions: Genetic screens for activator and repressor proteins
Lab: Learn to use pipetors

Yeast
Lab Intro
Pipeting
 L2.ppt
3
 9/22

Discussions: Xie et al.
Activity of MSEs from mid-sporulation promoters
Indentification of transcription factors that function at MSEs
Lab: Sterile Technique, Plate mutagenised yeast

 Xie et al.
 L3.ppt
4
 9/29

Discussions: Xie et a.l
Methods - lacZ reporter assays, Nortern blots, EMSA DNA binding assays,
Lab: Streak colonies to screen for silencing
Patching to screen for derepression

L4.ppt
5
 10/6 Discussions: Xie et a.l and Mead et al.
Methods - Silencing assays
Silencing vs repression, Sir2 vs Hst1
Lab: Screen for NT chimera mutants

Mead et al
McCord et al.
Sir2-Aging
Sir2 Review


6
 10/13 Lab: Perform filter lifts and lacZ assays
Analyze repression, silencing - pick mutant to sequence
Screen


7
 10/20

Lab: Spot culture dilutions for silencing assays
Analyze sequences, perform BLAST, map mutations on the structure


8
 10/27

Discussion: Results, problems and interpretations
Lab: Analyze sequences, perform BLAST, map mutations on the structure



L8.ppt
   
Experiment Protocol/analysis Data Links
LacZ Repression Assays LacZ assays LacZ data
Silencing Assays Silencing Assays Silencing Data
DNA Sequence Analysis DNA Analysis
WT SIR2::HST1 seq.
SIR2::HST1 Translation
Cn3D Instructions		 Mutant DNA sequence files
Cn3D structure file with Hst1 alignment
Download Cn3D program
9
 11/3 Lab: Analyze sequences, perform BLAST, map mutations on the structure DNA Analysis
 L9.ppt
10
 11/10 Discussions: Presentation of data, Interpretation of the results
Powerpoint files are due by 10:00 AM on 11/19		 Presentation Outline  

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last updated 5/17/2008